Immunohistochemistry & Microscopy
A HANDS-ON SHORT COURSE
Schedule and content are subject to change.
March 23-26, 2010.
Organizers: William Stahl, Eduardo Rosa-Molinar and Denis Baskin
This course is limited to 30 participants, so early registration is important.
Free wireless internet access on the MBL campus.
Short Course Registration is Now Closed
Day 1: Tuesday, March 23, 2010
Afternoon Particiapants arrive at MBL: registration and sleeping room assignment
3:15-4:15PM HCS/Journal of Histochemistry and Cystochemistry Business Meeting, OPTIONAL (open meeting)
5-6:30 PM Dinner at MBL
6:45-7:45 PM Evening Lecture: Ralph Albrecht, University of Wisconsin Madison; "Nanoparticle labels for co-localization at high spatial resolution" OPTIONAL
7:45-9:30 PM Poster Session and Annual Meeting Closing Reception
8:15 PM Orientation Session - William Stahl, The Histochemical Society and University of Washington
Day 2: Wednesday, March 24, 2010
7 AM Breakfast
8-8:15 AM Opening of session
8:15-9:15 AM Introduction to Immunihistochemistry • Charles Frevert, University of Washington
9:15-10 AM Lab Session
10-10:15 AM Refreshment break
10:15-10:45 AM Antibodies 1 - General Principles • Hinke Multhaupt, University of Copenhagen
10:45-11:15 AM Antigen Retrieval • Allen Gown, Phenopath
11:15-12 AM Lab Session
12-1 PM PM Lunch
1 - 2:30 PM Lab Session
2:30 - 3:15 PM Controls & Fixation • Denis Baskin, University of Washingotn and VA Puget Sound Medical Center
3:15-3:30 PM Refreshment break
3:30 - 4:30 PM Double Labeling/Localization Techniques • Gloria Hoffman, Morgan State University
4:30 - 6 PM Lab open
6 PM Dinner
7-8 Pm Optimization of Workflow and Throughput in a Modern Histology Core Environment •Laura Miller,, Leica Microsystems
8PM Lab Open
Day 3: Thursday, March 25, 2010
7-8 AM Breakfast
8-9:30 AM Lab Session
9:30-10 AM Antibodies 2 - Testing/Problems • Hinke Multhaupt, University of Copenhagen
10-11 AM Principles of Flourescence Microscopy • Margarida Barroso, Albany Medical College
11-12 AM Controlled Microwave Radiation is a Versatile Technology for Biological Sample Prepartion• Mark Sanders and Rick Giberson, University of Minnesota
12-1 PM Lunch
1:2:30PM Lab Session
2:30-3 PM Amplification Methods • Kevin Roth , University of Alabama at Birmingham
3-3:15 PM Refreshment break
3:15-6 PM Lab Session
6-7 PM Dinner
7-8 PM Molecular Labeling Technologies for Fluorescence Microscopy • Iain Johnson, Invitrogen/Molecular Probes
8 Pm Lab Open to complete work
Day 4: Friday, March 26, 2010
7-8 AM Breakfast, check out of sleeping rooms
8-9 AM Authentification of Images • Jerry Sedgewick
9-11 AM Wrap Up: questions, reports, troubleshooting • Irving Vega and Eduardo Rosa-Molinar
11 AM Depart MBL